Journal: Communications Biology

Article Title: Resistance to chemical carcinogenesis induction via a dampened inflammatory response in naked mole-rats

doi: 10.1038/s42003-022-03241-y

Figure Lengend Snippet: a Schematic diagram for carcinogenesis induction by intramuscular (i.m.) injection of 1 mg 3MC into the hind limb. b Kaplan–Meier curves of tumour-free mice and NMRs treated with 3MC. n = 9 animals per species. c Gross appearance and haematoxylin and eosin (HE) staining of a mouse tumour at 17 weeks and NMR muscle at 114 weeks after i.m. 3MC injection. Red arrows indicate injection sites. Scale bars: 1 cm (upper) and 50 μm (lower). d Schematic diagram for investigating short-term responses to 3MC after subcutaneous (s.c.) injection into the back skin. e Quantification of phospho-Histone H2A.X (pH2AX)-, 8-hydroxy-2′-deoxyguanosine (8-OHdG)-, and TUNEL-positive cells after s.c. 3MC-injection. f Immunohistochemical staining and quantification of Ki67-positive cells in NMR skin at 1 week after s.c. 3MC-injection. Red arrowheads show positive cells. Scale bar: 50 μm. For quantification of ( e and f ), data are presented as the mean ± SD of n = 3 animals. Log-rank test for ( b ). Unpaired t -test versus untreated control (Ctrl) for ( e and f ).

Article Snippet: Antigen retrieval was performed by heat-induced epitope retrieval in citrate buffer or Tris buffer, or by enzymatic retrieval using proteinase K . The sections were incubated with 1% bovine serum albumin in Tris-buffered saline with 0.1% NaN 3 for blocking, and stained with primary antibodies against CD45 (Abcam, ab10558), MPO (DAKO, A0398), IBA1 (FUJIFILM WAKO, 019-19741), CD3 (Nichirei, 413591), Ki67 (Abcam, ab16667), 8-OHdG (Santa Cruz Biotechnology, sc-393871), pH2AX (Cell Signalling Technology [CST], 9718), or HMGB1 (Abcam, ab79823).

Techniques: Injection, Staining, TUNEL Assay, Immunohistochemical staining

Journal: Communications Biology

Article Title: Resistance to chemical carcinogenesis induction via a dampened inflammatory response in naked mole-rats

doi: 10.1038/s42003-022-03241-y

Figure Lengend Snippet: a Schematic diagram for carcinogenesis assessment by DMBA/TPA treatment on the back skin. b Kaplan–Meier curves of tumour-free mice and NMRs after DMBA/TPA treatment. n = 6 animals per species. c Gross appearance and haematoxylin and eosin (HE) staining of mouse papillomas at 20 weeks and NMR skin at 55 weeks after starting DMBA/TPA treatment. Scale bars: 1 cm (upper) and 100 μm (lower). Inset is a higher magnification of NMR skin (scale bar: 50 μm). d Schematic diagram for investigating short-term responses to DMBA treatment on the back skin. e Quantification of phospho-Histone H2A.X (pH2AX)-positive cells at 24 h after DMBA treatment. f Schematic diagram for investigating short-term responses to DMBA/TPA treatment on the back skin. g Immunohistochemical staining and quantification of Ki67-positive cells in NMR skin at 2 weeks after starting DMBA/TPA treatment. Scale bar: 50 μm. For quantification of ( e and g ), data are presented as the mean ± SD of n = 4 (for mouse) or n = 3 (for NMR) animals. Log-rank test for ( b ). Unpaired t -test versus untreated control (Ctrl) for ( e and g ).

Article Snippet: Antigen retrieval was performed by heat-induced epitope retrieval in citrate buffer or Tris buffer, or by enzymatic retrieval using proteinase K . The sections were incubated with 1% bovine serum albumin in Tris-buffered saline with 0.1% NaN 3 for blocking, and stained with primary antibodies against CD45 (Abcam, ab10558), MPO (DAKO, A0398), IBA1 (FUJIFILM WAKO, 019-19741), CD3 (Nichirei, 413591), Ki67 (Abcam, ab16667), 8-OHdG (Santa Cruz Biotechnology, sc-393871), pH2AX (Cell Signalling Technology [CST], 9718), or HMGB1 (Abcam, ab79823).

Techniques: Staining, Immunohistochemical staining